702 Inhibition of class I HDACs preserves hair follicle inductivity in postnatal dermal cells

Induction of new hair follicles (HFs) may be an ultimate treatment goal for alopecia; however, functional cells with HF inductivity must expanded in bulk clinical use. In vitro culture conditions are completely different from the vivo microenvironment. Although fetal and postnatal dermal (DCs) have potential to induce HFs, they rapidly lose this during culture, accompanied by a drastic change gene expression. This suggests that epigenetic regulation involved. Of various histone deacetylases (HDACs), Class I HDACs noteworthy because ubiquitously expressed strongest deacetylase activity. study revealed DCs mice reduction is significant decrease H3 acetylation. However, MS-275, inhibitor class HDACs, preserves increasing alkaline phosphatase activity upregulating inductive genes such as BMP4, HEY1, WIF1. addition, inhibition activates Wnt signaling pathway, most well-described molecular pathway development, via increased acetylation within promoter region transcription factor LEF1. Our results suggest could target neogenesis HFs.